ABSTRACT
S. mombin is a plant used by traditional medical practitioners in the treatment of many diseases, both physical and mental. All experiments in this study were carried out using standard methods. In view of the medicinal potential of the stem bark of S. mombin, this study was therefore undertaken to evaluate the phytochemical constituents, the proximate compositions, antioxidant and antidepressant activities of the ethyl acetate extract of stem back of S. mombin. Phytochemical screening of stem back extract of S. mombin revealed the presence of alkaloids, cardiac glycosides, steroids, phenols, tannins, saponins, terpenoids and flavonoids. The proximate analysis of the crude stem back yielded 9.33 0.40%, 1.40 0.33%, 2.00 0.17%, 17.40 0.20%, 6.50 0.48% for moisture content, total ash, acid insoluble ash, water soluble ash, crude fat and crude fibre respectively. Free radical scavenging ability ranging from 47.74 – 80.18% and
79.28 – 88.29% was observed for ethyl acetate extract of stem back of S. mombin and ascorbic acid respectively. In the antidepressant study, the Forced Swim Test and Tail Suspension Test of the ethyl acetate extract of stem bark of S. mombin at (100 and
200mg/kg) showed a significant reduction in immobility time with 77.9% and 76.6% for Forced Swim Test and 65.7% and 91.5% for Tail Suspension Test. The result obtained proposed a sedative and antidepressant–like effect of ethyl acetate extract of stem bark of S. mombin, confirming the ethnomedicinal use of the stem bark of S. mombin for the management of depression.
CHAPTER ONE
INTRODUCTION AND LITERATURE REVIEW
1.1.0 INTRODUCTION
In recorded history, medicinal plants have been in use for the treatment of man and animal diseases (Osai, 1998 and Ibewike et al., 1997). A plant becomes a medicinal plant only when its biological activity has been ethnobotanically reported or scientifically established (Elujoba, 1997). In 1978, the World Health Organization (WHO) emphasized the importance of scientific research into herbal medicine. Many developing countries of the world look upon native medicinal plants as possible addition to the WHO’s list of ‘essential drugs’ once their values have been clinically proven. A simple first-principle biological technique in the evaluation of plant with known biological activity is to conduct a screening test (Elojoba, 1999). Various studies have been concerned worldwide in the identification and isolation of new therapeutic substances from diverse herbal plants to treat the existing and newly arousing diseases (Maria et al., 2016). S. mombin is one of such medicinal plant with a lot of potential, valuable, untapped resources of active drug for treating diseases (Ayoka, et al., 2008). Secondary metabolites are the classes of compounds which are known to display therapeutic activities against several sicknesses in man; they therefore explained the use of traditional therapeutic plants for the treatment of some diseases (Sabri et al., 2012). Unlike primary metabolites, secondary metabolites do not result in instant death, but rather in long-term weakening of the organism’s survivability, fertility, or aesthetics, or perhaps in no significant change at all (Wikipedia, 2018).
S. mombim linn is a small tree that grows up to 20m (60 ft) high and 1.5 m (5 ft.) in girth, moderately buttressed; stem bark is thick, corky, deeply fissured, slash pale pink, darkening rapidly, branchlets glabrous; leaves pinnate, leaflets 5-8 opposite pairs with a terminal leaflet. It belongs to the family Anarcardiaceae. Its flowers develop between January – May and fruits between July – September. The fruits have a sharp, somewhat acid taste and are edible. The matured fruit has a leathery skin and a thin
layer of pulp. The fruit pulp is either eaten fresh, or made into juice, concentrate, jellies, and sherbets. The fruit-juice is used as a febrifuge and diuretic. The roots are also used as febrifuge in Ivory Coast (Ayoka, 2006). The bark is used as a purgative and in local applications in the treatment of leprosy. The stem bark decoction is also used in the treatment of severe cough. It serves as an emetic, a remedy for diarrhea, dysentery, hemorrhoids and a treatment for gonorrhea and leucorrhoea (Ayoka, 2006). The decoction of the astringent stem bark is believed to expel calcifications from the bladder. The juice of crushed leaves and the powder of dried leaves are used on wounds and inflammations. The gum is employed as an expectorant and to expel tapeworms (Rodriguez, 2000). A decoction of the mashed leaves is used by the Ibos (Nigeria) for washing a swollen face. A leave infusion is a common cough remedy or used as a laxative for fever with constipation. A leave decoction is used in treatment of gonorrhea. The leaves are used in Ivory Coast for fresh wounds to prevent inflammation (Igbokwe,
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